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Running CodeSet Assays on the nCounter® Pro/MAX/FLEX

This course covers the reagents, set-up, and instrument steps of a CodeSet assay run on the nCounter MAX/FLEX/Pro. Let's get started!

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What To Expect And Course Objectives

Welcome to the nCounter Analysis System training course! This course will teach you everything you need to know to run your nCounter® Analysis System. Each lesson is comprised of a video showing you the steps in the workflow and helpful documents that relate to the video content. You are welcome to download the manuals in each section and save them for your convenience.

Course objectives:

  1.  Understand how the CodeSet chemistry works
  2.  Learn how to use the nCounter Analysis System to process your samples
  3.  Know how to maintain and troubleshoot the instrument
  4.  Check your understanding throughout the course with short quizzes!

Now let's get started!

Curriculum66 min

  • Module 1: Introduction

    This course will cover the steps needed to perform the assay for NanoString nCounter MAX gene expression panels.

  • Introduction to the nCounter® Analysis System Training Series 1 min
  • Preparing RNA From FFPE or Fresh Frozen Samples
  • Preparing Cell Lysate Samples
  • Module 2: Master Kit Consumables
  • Master Kit Consumables 3 min
  • Knowledge Check! 2 min
  • Module 3: CodeSet Hybridization Setup
  • CodeSet Hybridization Setup Protocol 1 min
  • CodeSet Hybridization Setup 6 min
  • Knowledge Check! 3 min
  • Module 4: Setting Up and Running the nCounter Prep Station
  • MAN-C0035 nCounter MAX/FLEX Analysis System User Manual
  • nCounter Pro Analysis System User Manual
  • Setting Up the nCounter® Prep Station 13 min
  • Knowledge Check! 3 min
  • Module 5: Loading and Operating the nCounter Digital Analyzer
  • MAN-C0035 nCounter MAX/FLEX Analysis System User Manual
  • nCounter Pro Analysis System User Manual
  • nCounter® File Definitions 13 min
  • Knowledge Check! 2 min
  • Loading and Operating the nCounter® Digital Analyzer 6 min
  • Knowledge Check! 2 min
  • Downloading Data From The Digital Analyzer 2 min
  • Module 6: nCounter® Analysis System Maintenance and Troubleshooting
  • Conditioning The O-Rings On The nCounter® Prep Station 3 min
  • Knowledge Check! 1 min
  • MK0673_MAX-FLEX-download_log_Instructions.pdf
  • Downloading Logs From The nCounter® Analysis System 2 min
  • Knowledge Check! 1 min
  • Feedback Survey
  • Feedback Survey 2 min

What To Expect And Course Objectives

Welcome to the nCounter Analysis System training course! This course will teach you everything you need to know to run your nCounter® Analysis System. Each lesson is comprised of a video showing you the steps in the workflow and helpful documents that relate to the video content. You are welcome to download the manuals in each section and save them for your convenience.

Course objectives:

  1.  Understand how the CodeSet chemistry works
  2.  Learn how to use the nCounter Analysis System to process your samples
  3.  Know how to maintain and troubleshoot the instrument
  4.  Check your understanding throughout the course with short quizzes!

Now let's get started!

Curriculum66 min

  • Module 1: Introduction

    This course will cover the steps needed to perform the assay for NanoString nCounter MAX gene expression panels.

  • Introduction to the nCounter® Analysis System Training Series 1 min
  • Preparing RNA From FFPE or Fresh Frozen Samples
  • Preparing Cell Lysate Samples
  • Module 2: Master Kit Consumables
  • Master Kit Consumables 3 min
  • Knowledge Check! 2 min
  • Module 3: CodeSet Hybridization Setup
  • CodeSet Hybridization Setup Protocol 1 min
  • CodeSet Hybridization Setup 6 min
  • Knowledge Check! 3 min
  • Module 4: Setting Up and Running the nCounter Prep Station
  • MAN-C0035 nCounter MAX/FLEX Analysis System User Manual
  • nCounter Pro Analysis System User Manual
  • Setting Up the nCounter® Prep Station 13 min
  • Knowledge Check! 3 min
  • Module 5: Loading and Operating the nCounter Digital Analyzer
  • MAN-C0035 nCounter MAX/FLEX Analysis System User Manual
  • nCounter Pro Analysis System User Manual
  • nCounter® File Definitions 13 min
  • Knowledge Check! 2 min
  • Loading and Operating the nCounter® Digital Analyzer 6 min
  • Knowledge Check! 2 min
  • Downloading Data From The Digital Analyzer 2 min
  • Module 6: nCounter® Analysis System Maintenance and Troubleshooting
  • Conditioning The O-Rings On The nCounter® Prep Station 3 min
  • Knowledge Check! 1 min
  • MK0673_MAX-FLEX-download_log_Instructions.pdf
  • Downloading Logs From The nCounter® Analysis System 2 min
  • Knowledge Check! 1 min
  • Feedback Survey
  • Feedback Survey 2 min